Zhongguo shi yan xue ye xue za zhi, 2007-06, Vol.15 (3), p.449
2007
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Details
- Autor(en) / Beteiligte
- Titel
- Expression and role of toll-like receptors in U937 cells
- Ist Teil von
- Zhongguo shi yan xue ye xue za zhi, 2007-06, Vol.15 (3), p.449
- Ort / Verlag
- China
- Erscheinungsjahr
- 2007
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- The aim of study was to explore the potential application of targeting at Toll-like receptors (TLRs) in the immunotherapy of acute myelocytic leukemia, and to investigate the expression of TLR and the effects of TLR 8 agonist ssRNA40/LyoVec on proliferation, apoptosis and cell cycle of U937 cells. The expression of TLR 1 - 9 in U937 cells was detected by using reverse transcription polymerase chain reaction (RT-PCR) and the expression of TLR 8 was assayed by flow cytometry (FCM). The effect of TLR 8 agonist, ssRNA40/LyoVec, at different concentrations on U937 cells proliferation was evaluated by CCK-8, apoptosis and cell cycle were detected by FCM. The results showed that U937 cells expressed TLR 1 - 9. TLR 8 agonist ssRNA40/LyoVec could inhibit the growth of U937 cells both in time-and dose-dependent manner and the inhibitory rate could reach 70%. It also increased the percentage of cells in G(0)/G(1) phase. There was no significant difference in percentage of apoptotic cells between control and treated grou
- Sprache
- Chinesisch
- Identifikatoren
- ISSN: 1009-2137Titel-ID: cdi_pubmed_primary_17605842
- Format
- –
- Schlagworte
- Apoptosis - drug effects, Cell Cycle - drug effects, Cell Proliferation - drug effects, Dose-Response Relationship, Drug, Humans, Leukemia, Myeloid, Acute - metabolism, Leukemia, Myeloid, Acute - pathology, Reverse Transcriptase Polymerase Chain Reaction, Toll-Like Receptor 8 - agonists, Toll-Like Receptors - metabolism, U937 Cells