Details

Autor(en) / Beteiligte

• Kapaki, Elisabeth
• Liappas, Ioannis
• Lyras, Leonidas
• Paraskevas, George P
• Mamali, Ioanna
• Theotoka, Ioulia
• Bourboulis, Nikolaos
• Liosis, Ioannis
• Petropoulou, Olga
• Soldatos, Konstantinos

Titel

Oxidative Damage to Plasma Proteins in Patients with Chronic Alcohol Dependence: The Effect of Smoking

Ist Teil von

• In vivo (Athens), 2007-05, Vol.21 (3), p.523

Ort / Verlag

Greece: International Institute of Anticancer Research

Erscheinungsjahr

2007

Quelle

Free E-Journal (出版社公開部分のみ）

Beschreibungen/Notizen

• Background: Accumulating evidence implicates oxidative stress in ethanol-induced toxicity. Ethanol has been reported to be involved in oxidative damage, mostly in vitro, or in post mortem tissues, while biochemical abnormalities in the blood or serum are scanty or lacking. The aim of the present study was to examine the oxidative status of plasma proteins as markers of oxidative stress in subjects with chronic alcohol dependence (CAD). Since smoking has also been associated with oxidative stress this factor was also considered. Patients and Methods: A total of 71 patients with CAD and 61 healthy volunteers of comparable age were included in the study. The protein carbonyl assay was carried out in plasma, as a reliable measure of general oxidative protein damage, in these two groups. Results: Increased plasma protein carbonyls (PCs) were found in patients with CAD as compared with the control group [mean values (nmol/mg protein): 4.73±1.46 and 3.62±0.91 respectively, p<0.000001]. Within the control group, smokers had higher PCs than the non-smokers, however this difference was of marginal significance [mean values (nmol/mg protein): 3.93±1.32 and 3.47±0.63, respectively]. The CAD group had significantly increased PCs compared with both the smoker and the non-smoker subgroups of the controls (p<0.001 and p<0.0001, respectively). Duration of alcohol consumption, daily alcohol intake, smoke load, folic acid and vitamin B12 levels did not correlate significantly with PC levels. Conclusion: The above results support the evidence for systemic oxidative stress in CAD, which must be attributed mainly to alcohol consumption, while smoking may act synergistically.