Zhongguo shi yan xue ye xue za zhi, 2007-04, Vol.15 (2), p.337
2007
Volltextzugriff (PDF)
Details
- Autor(en) / Beteiligte
- Titel
- Application of chimerism analysis to allogeneic hematopoietic stem cell transplantation by STR-PCR
- Ist Teil von
- Zhongguo shi yan xue ye xue za zhi, 2007-04, Vol.15 (2), p.337
- Ort / Verlag
- China
- Erscheinungsjahr
- 2007
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- The aim of this study was to analyze chimerism, evaluate the status of engraftment and predict the outcome of allogeneic hematopoietic stem cell transplantation (allo-HSCT) by multiple short tandem repeat (STR) amplification using fluorescence labeling polymerase chain reaction (PCR) combined with capillary electrophoresis. Peripheral blood and bone marrow in 27 patients who received myeloablative allogenetic cell transplantation were collected before and after transplantation in different times. 10 and 7 different STR markers were co-amplified in a single reaction by using a commercial AmpF/STR Profiler Plus/Cofiler plus PCR amplification kits. Separation of the PCR products and fluorescence detection were performed by ABI prism 310 Genetic Analyzer with capillary electrophoresis. The Genescan and Genotype soft ware were used for size calling and quantification of peak areas. The formula to calculate donor chimerism values was based on the different allelic distribution type between donor and recipient. The
- Sprache
- Chinesisch
- Identifikatoren
- ISSN: 1009-2137Titel-ID: cdi_pubmed_primary_17493343
- Format
- –
- Schlagworte
- Adolescent, Adult, Female, Graft vs Host Disease - prevention & control, Humans, Leukemia, Myelogenous, Chronic, BCR-ABL Positive - therapy, Leukemia, Myeloid, Acute - therapy, Male, Peripheral Blood Stem Cell Transplantation - methods, Polymerase Chain Reaction - methods, Recurrence, Tandem Repeat Sequences, Transplantation Chimera, Transplantation, Homologous