Details

Autor(en) / Beteiligte

• Mancuso, Katherine
• Hendrickson, Anita E
• Connor, Jr, Thomas B
• Mauck, Matthew C
• Kinsella, James J
• Hauswirth, William W
• Neitz, Jay
• Neitz, Maureen

Titel

Recombinant adeno-associated virus targets passenger gene expression to cones in primate retina

Ist Teil von

• Journal of the Optical Society of America. A, Optics, image science, and vision, 2007-05, Vol.24 (5), p.1411

Ort / Verlag

United States

Erscheinungsjahr

2007

Quelle

MEDLINE

Beschreibungen/Notizen

• Recombinant adeno-associated virus (rAAV) is a promising vector for gene therapy of photoreceptor-based diseases. Previous studies have demonstrated that rAAV serotypes 2 and 5 can transduce both rod and cone photoreceptors in rodents and dogs, and it can target rods, but not cones in primates. Here we report that using a human cone-specific enhancer and promoter to regulate expression of a green fluorescent protein (GFP) reporter gene in an rAAV-5 vector successfully targeted expression of the reporter gene to primate cones, and the time course of GFP expression was able to be monitored in a living animal using the RetCam II digital imaging system.