Sichuan da xue xue bao. Journal of Sichuan University. Yi xue ban, 2007-01, Vol.38 (1), p.35
2007
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- Autor(en) / Beteiligte
- Titel
- An experimental research on tumor metastasis treated by chicken homologous matrix metalloproteinase-2 vaccine combined with low-dose cisplatin
- Ist Teil von
- Sichuan da xue xue bao. Journal of Sichuan University. Yi xue ban, 2007-01, Vol.38 (1), p.35
- Ort / Verlag
- China
- Erscheinungsjahr
- 2007
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- To evaluate the cooperating effect on whether the chicken homologous matrix metalloproteinase-2 (c-MMP-2) vaccine combined with low-dose cisplatin (DDP) can enhance the treatment efficacy of tumor metastasis. The eukaryotic expression plasmid encoding chicken homologous MMP-2 was constructed by recombinant DNA technique. In this experiment, the lung metastasis model of murine colon adenocarcinoma (C26) was established in 6- to 8- weeks of age female BALB/c mice, and then treated with 0.9% NaCl solution (NS), DDP, c-MMP-2, or c-MMP-2 combined DDP. The number of tumor metastasis nodules on murine lung surface was counted and the lungs were weighed after the completion of above treatments. The tumor microvessel density (MVD) and cell apoptosis were evaluated by immunohistochemical and TUNEL methods. The titer and type of autoantibodies against MMP-2 in serum of mice were evaluated by enzyme-linked immunosorbent assay (ELISA). Compared with three other control groups, the combined treatment group significantly de
- Sprache
- Chinesisch
- Identifikatoren
- ISSN: 1672-173XTitel-ID: cdi_pubmed_primary_17294723
- Format
- –
- Schlagworte
- Animals, Apoptosis - drug effects, Apoptosis - immunology, Autoantibodies - immunology, Cancer Vaccines - genetics, Cancer Vaccines - immunology, Cell Line, Tumor, Chickens, Cisplatin - pharmacology, Cisplatin - therapeutic use, Drug Resistance, Neoplasm - immunology, Female, Immunoconjugates - immunology, Immunoconjugates - pharmacology, Lung - drug effects, Lung - immunology, Lung - pathology, Matrix Metalloproteinase 2 - genetics, Matrix Metalloproteinase 2 - immunology, Mice, Mice, Inbred BALB C, Neoplasm Metastasis - drug therapy, Neoplasm Metastasis - immunology, Neoplasm Metastasis - physiopathology, Neovascularization, Pathologic - drug therapy, Neovascularization, Pathologic - immunology, Sequence Homology, Nucleic Acid, Vaccines, DNA - genetics, Vaccines, DNA - immunology