Zhonghua yi xue yi chuan xue za zhi, 2007-02, Vol.24 (1), p.42
2007
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Details
- Autor(en) / Beteiligte
- Titel
- Expression of fusion gene PAX3/PAX7-FKHR and chromosomal aberration in rhabdomyosarcoma
- Ist Teil von
- Zhonghua yi xue yi chuan xue za zhi, 2007-02, Vol.24 (1), p.42
- Ort / Verlag
- China
- Erscheinungsjahr
- 2007
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- To detect the PAX3/PAX7-FKHR fusion transcripts to identify genetic alteration in embryonal rhabdomyosarcoma (ERMS) and alveolar rhabdomyosarcoma (ARMS) tissues. One-step reverse transcription- polymerase chain reaction (RT-PCR) were used to detect the expression of the PAX3/PAX7-FKHR fusion transcrips in 16 cases of rhabdomyosarcoma (7 cases of ARMS, 9 cases of ERMS) and 16 specimens were compared to the surrounding normal tissue. Comparative genomic hybridization (CGH) was employed to detect the genomic imbalance (DNA loss or amplification) in 16 RMS cases. PAX3-FKHR fusion transcripts were positive in 3/7 and PAX 7-FKHR fusion transcripts were positive in 2/7 of ARMS patients, respectively, and were all negative in ERMS and Control tumors. There were different chromosome variations for each RMS, chromosome amplification was frequently seen in 1p36 (69%), 5q32 (56%), 8q21 (63%), 13q14 (69%), 19q (63%), 20q (56%). Chromosome loss was frequently seen in 3p21-pter (56%), 9p23-pter (50%), 10q (69%), 16/16q24 (5
- Sprache
- Chinesisch
- Identifikatoren
- ISSN: 1003-9406Titel-ID: cdi_pubmed_primary_17285543
- Format
- –
- Schlagworte
- Chromosome Aberrations, Comparative Genomic Hybridization, Forkhead Box Protein O1, Forkhead Transcription Factors - genetics, Gene Expression Regulation, Neoplastic, Humans, Oncogene Proteins, Fusion - genetics, Paired Box Transcription Factors - genetics, PAX3 Transcription Factor, PAX7 Transcription Factor - genetics, Reverse Transcriptase Polymerase Chain Reaction, Rhabdomyosarcoma - genetics