Bioscience, biotechnology, and biochemistry, 2007-02, Vol.71 (2), p.520-526
2007
Volltextzugriff (PDF)
Details
- Autor(en) / Beteiligte
- Titel
- Expression of rice (Oryza sativa L. var. Nipponbare) alpha-galactosidase genes in Escherichia coli and characterization
- Ist Teil von
- Bioscience, biotechnology, and biochemistry, 2007-02, Vol.71 (2), p.520-526
- Ort / Verlag
- Tokyo: Japan Society for Bioscience, Biotechnology, and Agrochemistry
- Erscheinungsjahr
- 2007
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Two putative alpha-galactosidase genes from rice (Oryza sativa L. var. Nipponbare) belonging to glycoside hydrolase family 27 were cloned and expressed in Escherichia coli. These enzymes showed alpha-galactosidase activity and were purified by Ni Sepharose column chromatography. Two purified recombinant alpha-galactosidases (alpha-galactosidase II and-III; alpha-Gal II and III) showed a single protein band on SDS-PAGE with molecular mass of 42 kDa. These two enzymes cleaved not only alpha-D-galactosyl residues from the non-reducing end of substrates such as melibiose, raffinose, and stachyose, but also liberated the galactosyl residues attached to the O-6 position of the mannosyl residue at the reducing-ends of mannobiose and mannotriose. In addition, these enzymes clipped the galactosyl residues attached to the inner-mannosyl residues of mannopentaose. Thus, alpha-Gal II catalyzes efficient degalactosylation of galactomannans, such as guar gum and locust bean gum.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0916-8451eISSN: 1347-6947DOI: 10.1271/bbb.60554Titel-ID: cdi_pubmed_primary_17284836
- Format
- –
- Schlagworte
- ALFA GALACTOSIDASA, ALPHA GALACTOSIDASE, alpha-Galactosidase - biosynthesis, alpha-Galactosidase - genetics, Amino Acid Sequence, Biological and medical sciences, CLONACION MOLECULAR, CLONAGE MOLECULAIRE, Cloning, Molecular, Crystallography, X-Ray, Databases, Genetic, DNA Primers, Escherichia coli, Escherichia coli - enzymology, Escherichia coli - genetics, EXPRESION GENICA, EXPRESSION DES GENES, Fundamental and applied biological sciences. Psychology, galactomannans, galactomanno-oligosaccharides, GENE EXPRESSION, Gene Expression Regulation, Enzymologic - physiology, MANANOS, MANNANE, MANNANS, MOLECULAR CLONING, Molecular Sequence Data, OLIGOSACARIDOS, OLIGOSACCHARIDE, OLIGOSACCHARIDES, Oryza - enzymology, Oryza - genetics, ORYZA SATIVA, Recombinant Proteins - chemistry, Recombinant Proteins - isolation & purification, Reverse Transcriptase Polymerase Chain Reaction, rice (Oryza sativa L. var. Nipponbare), Substrate Specificity, α-galactosidase