Details

Autor(en) / Beteiligte

• Chancerelle, Y
• Alban, C
• Viret, R
• Tosetti, F
• Kergonou, J F

Titel

Immunological relevance of malonic dialdehyde (MDA): IV. Further evidences about the epitope recognized by antibodies obtained from rabbits immunized with MDA-modified lysozyme

Ist Teil von

• Biochemistry international, 1991-05, Vol.24 (1), p.157

Ort / Verlag

Australia

Erscheinungsjahr

1991

Quelle

MEDLINE

Beschreibungen/Notizen

• Reactions of MDA with primary amino groups produce inter- or intra-molecular 1-amino-3-imino-propene (AIP) bridges, leading to structural modifications of biological molecules. In this work, applying electrophoresis followed by transfer onto nitrocellulose membranes, we observed that serum of a rabbit immunized with MDA-modified lysozyme (ML) reacts not only with ML and native lysozyme (L), but also with MDA-modified ribonuclease, cytochrome c or polylysine (MR, MC and MP respectively), while it does not react with native ribonuclease, cytochrome c or polylysine (R, C and P respectively). These results confirm previous ones indicating that sera of rabbits immunized with ML contain antibodies reacting specifically with epitopes containing AIP bridges.