Details

Autor(en) / Beteiligte

• Liu, Peng
• Li, Da-Jiang
• Qin, Hai-De
• Zhang, Ru-Hua
• Chen, Li-Zhen
• Zeng, Yi-Xin

Titel

Screening for mutations in the hotspot mutation regions of PIK3CA gene in nasopharyngeal carcinoma

Ist Teil von

• Ai zheng = Aizheng = Chinese journal of cancer, 2007-01, Vol.26 (1), p.15

Ort / Verlag

China

Erscheinungsjahr

2007

Quelle

MEDLINE

Beschreibungen/Notizen

• Recent studies showed high frequency of phosphatidylinositol 3-kinase catalytic alpha polypeptide (PIK3CA) mutations in various human cancers; notably, these mutations frequently locate in the hotspot mutation regions of PIK3CA exon 9 and exon 20 with functional significance in tumorigenesis, invasion, and anti-apoptosis. This study was to screen for mutations in the hotspot mutation regions of PIK3CA in nasopharyngeal carcinoma (NPC), and explore the correlation of PIK3CA mutations to tumorigenesis of NPC. PIK3CA exon 9 and exon 20 in 46 specimens of sporadic primary NPC tissues were screened by polymerase chain reaction (PCR)-clone sequencing; those in 46 samples of matched NPC peripheral blood and 3 NPC cell lines CNE1, CNE2, and SUNE1 were directly sequenced. Among the 46 specimens of NPC, 2 (4.3%) had point mutation in PIK3CA exon 9 [T1563G (521Asn-->Lys) and A1646G (549Asp-->Gly)], 18 had multiple mutations in PIK3CA exon 9 (A1634C-G1658C-del 1659T), which might be the homologous sequence of Cat Eye Syn