Details

Autor(en) / Beteiligte

• Wu, Yinliang
• Liu, Suying
• Hou, Dongiun
• Shen, Jianzhong
• Wang, Hai
• Shan, Jihao

Titel

Determination of residues of three stilbene drugs in animal tissues using gas chromatography-mass spectrometry

Ist Teil von

• Sepu, 2006-09, Vol.24 (5), p.462

Ort / Verlag

China

Erscheinungsjahr

2006

Quelle

MEDLINE

Beschreibungen/Notizen

• A method has been developed to determine residual stilbenes such as diethylstilbestrol (DES), dienestrol (DIS) and hexestrol (HS) in animal tissues using solid phase extraction (SPE) and gas chromatography-mass spectrometry (GC-MS). The procedures for extraction, cleanup on an LC-Si solid phase extraction cartridge and derivatization of stilbenes were established and optimized. The analytes were detected by mass spectrometer with electron impact source in selected ion monitoring mode (EI/SIM), and quantified with an external standard calibration curve method. Linear calibration curves were obtained in the concentration ranges from 5 to 500 microg/L for HS and from 10 to 1000 microg/L for DES and DIS (the correlation coefficients were above 0.99). Recoveries of the stilbenes were 73.0%-86.5%, and the relative standard deviations were between 1.0% and 7.2%. The limits of detection were 0.30 microg/kg for cis-DES, 0.10 microg/kg for trans-DES and HS and 0.15 microg/kg for DIS in pork and swine liver.