Details

Autor(en) / Beteiligte

• Liu, De-Yu
• Wu, Yan-Wan

Titel

Meiosis related gene expression in rat spermatogenesis

Ist Teil von

• Zhonghua nan ke xue, 2006-10, Vol.12 (10), p.883

Ort / Verlag

China

Erscheinungsjahr

2006

Quelle

MEDLINE

Beschreibungen/Notizen

• To find the expression of specific genes related to the meiosis of germ cells during spermatogenesis in the rat testis. Segments of seminiferous tubules were obtained from the adult male SD rats, at stages XIII - I of meiosis, and the interstitial cells of the same testis were isolated under the stereomicroscope. The total RNAs of stages XIII - I segments and the testicular interstitial cells were extracted respectively, and mRNA differential display RT-PCR (DDRT-PCR) was conducted. The obtained cDNA fractions were purified and recovered, the reverse dot blot hybridization, sub-clones and screens of blue/white dots performed, and the results of sub-clones were identified by restriction endonuclease EcoR I digestion. Sixteen differential cDNA fractions were obtained through primary DDRT-PCR, 7 from stages XIII - I segments and 9 from the testicular interstitial cells. Another 11 were selected for further screening by reverse dot blot hybridization, their size ranging from 200 to 500 bp, of which 6 were from th