Wei sheng wu hsüeh pao, 2006-08, Vol.46 (4), p.531
2006
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- Autor(en) / Beteiligte
- Titel
- Analysis of mechanism and relationship of GacA and RsmA, two regulators of antibiotics production in Pseudomonas sp. M18
- Ist Teil von
- Wei sheng wu hsüeh pao, 2006-08, Vol.46 (4), p.531
- Ort / Verlag
- China
- Erscheinungsjahr
- 2006
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- In previous study, it has already been confirmed that the wild type strain of Pseudomonas sp. M18 isolated from the agricultural soil can produce two antifungal agents phenazine-1-carboxylic acid (PCA) and pyoluteorin (Plt). Biosynthesis and secretion of these secondary metabolites contribute to its biological control and suppression of soilborne pathogenic fungi. As main regulators, GacA and RsmA differentially exert global regulation on production of PCA and Plt, respectively. In order to study the regulatory mechanism of secondary metabolites production in Pseudomonas sp. M18, a gacArsmA double mutant, designated as M18GR, was constructed with insertional mutation. Then, the mutant M18G, M18R, M18GR and the wild type strain M18 were inoculated into PPM or King's medium B (KMB), respectively. During cultivation of strain M18 and its derivatives, their PCA and Pit were respectively detected with High Performance Liquid Chromatography (HPLC). The results showed that PCA production in the mutant M18GR was lowe
- Sprache
- Chinesisch
- Identifikatoren
- ISSN: 0001-6209Titel-ID: cdi_pubmed_primary_17037049
- Format
- –
- Schlagworte
- Antifungal Agents - metabolism, Bacterial Proteins - genetics, Bacterial Proteins - metabolism, Blotting, Southern, Chromatography, High Pressure Liquid, Gene Expression Regulation, Bacterial - genetics, Gene Expression Regulation, Bacterial - physiology, Mutagenesis, Insertional, Phenazines - metabolism, Phenols - metabolism, Pseudomonas - genetics, Pseudomonas - growth & development, Pseudomonas - metabolism, Pyrroles - metabolism