Sie befinden Sich nicht im Netzwerk der Universität Paderborn. Der Zugriff auf elektronische Ressourcen ist gegebenenfalls nur via VPN oder Shibboleth (DFN-AAI) möglich. mehr Informationen...
Influence and mechanism of peroxisome proliferation activated receptor-alpha expression induced by WY14643 in rat lung with acute lung injury
Ist Teil von
Zhongguo wei zhong bing ji jiu yi xue, 2006-08, Vol.18 (8), p.466
Ort / Verlag
China
Erscheinungsjahr
2006
Link zum Volltext
Quelle
MEDLINE
Beschreibungen/Notizen
To investigate the effect of WY14643 [peroxisome proliferation activated receptor-alpha (PPAR-alpha) activator, 4-cholro-6-(2.3-xylidino)-2-pyrimidinylthio acetic acid] on PPAR-alpha expression in lung of acute lung injury (ALI) induced by lipopolysaccharide (LPS) and its mechanism.
One hundred and four male Wistar rats were randomly divided into four groups: control group, ALI group, WY14643 1 mg group and WY14643 3 mg group. Wistar rats were given LPS (5 mg/kg) before intratracheal challenge with WY14643 (1 mg/kg and 3 mg/kg). Rats of all groups were sacrificed at 1, 2, 4, 8 hours after LPS challenge. The levels of PPAR-alpha mRNA in lung were measured by semi quantitative reverse transcription-polymerase chain reaction, the levels of PPAR-alpha protein in rats lung homogenate were measured by Western blot.
The levels of PPAR-alpha mRNA at 2,4 and 8 hours in WY14643 1 mg group and WY14643 3 mg group were significantly higher than that in ALI group (P<0.05 or P<0.01). The levels of PPAR-alpha mRNA at all tim