Chung-hua nei kʿo tsa chih, 2006-05, Vol.45 (5), p.396
2006
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- Autor(en) / Beteiligte
- Titel
- Inhibition of hepatitis B virus S gene and C gene expression by different 10-23 DNAzymes substrate-recognition domains
- Ist Teil von
- Chung-hua nei kʿo tsa chih, 2006-05, Vol.45 (5), p.396
- Ort / Verlag
- China
- Erscheinungsjahr
- 2006
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- To explore the inhibition effects of 10-23 DNAzymes with different substrate-recognition domains targeting hepatitis B virus (HBV) S gene and C gene expression in 2.2.15 cells. 10-23 DNAzymes with different substrate-recognition domains specific to HBV S gene open reading frame (ORF) A(157)UG and HBV C gene ORF A(1816)UG were designed and synthesized, respectively. Different 10-23 DNAzymes were transfected into 2.2.15 cells which is a stable HBV producing cell line. HBsAg and HBeAg secreted into culture media were detected by radioimmunoassay (RIA) and HBV DNA levels were measured by real-time PCR. 3-(4, 5-dimethylthiagol-2-yl)-2, 5-drphnyl tetrazolium bromide (MTT) assays were performed to evaluate cytotoxicity. HBsAg and HBeAg expressions were reduced by various DNAzymes (0.1 - 2.5 micromol/L) with different substrate-recognition domains after transfection. The antiviral effects of DNAzymes were apparent until 72 h post-transfection. The inhibition rates of the DNAzymes at the same dose on HBsAg and HBeAg i
- Sprache
- Chinesisch
- Identifikatoren
- ISSN: 0578-1426Titel-ID: cdi_pubmed_primary_16780744
- Format
- –
- Schlagworte
- Cell Line, DNA, Catalytic - pharmacology, DNA, Viral - analysis, Dose-Response Relationship, Drug, Gene Expression Regulation, Viral - drug effects, Genes, Viral, Hepatitis B e Antigens - analysis, Hepatitis B Surface Antigens - analysis, Hepatitis B virus - drug effects, Hepatitis B virus - genetics, Humans, Polymerase Chain Reaction, Transfection