Zhonghua jiehe he huxi zazhi, 2006-03, Vol.29 (3), p.171
2006
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- Autor(en) / Beteiligte
- Titel
- Effects of immunotherapy on the expression of costimulatory molecules on dendritic cells in a mouse model of asthma
- Ist Teil von
- Zhonghua jiehe he huxi zazhi, 2006-03, Vol.29 (3), p.171
- Ort / Verlag
- China
- Erscheinungsjahr
- 2006
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- To establish a mouse model of ovalbium in (OVA) specific immunotherapy, and to explore the effects of specific immunotherapy on the expression of CD(80) and CD(86) on spleen-derived dendritic cells (DC) in a mouse asthmatic model. A hundred and twenty BALB/c mice were randomly divided into three groups, with 40 mice each. The asthma model (group A) was established by intraperitoneal injection of 10 microg OVA and 1% OVA aerosol challenges. Consecutive subcutaneous injection of 1 mg OVA in the dorsal aspect of the foot was then given to establish the model of OVA specific immunotherapy (group B), while PBS (0.01 mol/L) was given in group C to serve as the control. The pathological changes of lung tissues were studied by HE stain. The serum OVA-specific IgE level, and IL-2 and IL-4 production in the splenic T cells were determined by enzyme-linked immunosorbent assay (ELISA). The expression of CD(80) and CD(86) on DC, which were isolated from the spleen, were detected by fluorescein-activated cell sorter. (3)H-
- Sprache
- Chinesisch
- Identifikatoren
- ISSN: 1001-0939Titel-ID: cdi_pubmed_primary_16677480