Biofizika, 2006-03, Vol.51 (2), p.282
2006
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- Autor(en) / Beteiligte
- Titel
- Covalent binding of 1,N(6)-ethenoadenosine diphosphate to catalytic and noncatalytic sites of chloroplast ATP-synthase
- Ist Teil von
- Biofizika, 2006-03, Vol.51 (2), p.282
- Ort / Verlag
- Russia (Federation)
- Erscheinungsjahr
- 2006
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- The catalytic and noncatalytic sites of the chloroplast coupling factor (CF1) were selectively modified by incubation with the dialdehyde derivative of the fluorescent adenosine diphosphate analogue 1,N(6)-ethenoadenosine diphosphate. The modified CF1 was reconstituted with EDTA-treated chloroplast thylakoid membranes. The influence of light-induced transmembrane proton gradient and of phosphate ions on the fluorescence of 1,N(6)-ethenoadenosine diphosphate covalently bound to catalytic sites of reconstituted CF1 (ATP-synthase) was studied. Upon illumination of thylakoid membranes with saturating white light, the quenching of fluorescence of covalently bound 1,N(6)-ethenoadenosine diphosphate was observed. The quenching was reversed by the addition of inorganic phosphate to the reaction mixture in the dark. Repeated illumination induced the quenching once again: however, the addition of phosphate ions did not affect the fluorescence intensity now. When 1,N(6)-ethenoadenosine diphosphate was covalently bound to noncatalytic sites of ATP-synthase, no similar fluorescent changes were observed. The interrelation of the observed changes of 1,N(6)-ethenoadenosine diphosphate fluorescence and the mechanism of energy-dependent changes in the structure of the catalytic site of ATP-synthase is discussed.
- Sprache
- Russisch
- Identifikatoren
- ISSN: 0006-3029Titel-ID: cdi_pubmed_primary_16637334
- Format
- –
- Schlagworte
- Adenosine Diphosphate - analogs & derivatives, Adenosine Diphosphate - chemistry, Adenosine Diphosphate - pharmacology, Adenosine Triphosphate - chemistry, Adenosine Triphosphate - metabolism, Catalytic Domain - drug effects, Chloroplast Proton-Translocating ATPases - antagonists & inhibitors, Chloroplast Proton-Translocating ATPases - chemistry, Chloroplast Proton-Translocating ATPases - metabolism, Fluorescent Dyes - chemistry, Fluorescent Dyes - pharmacology, Light, Pisum sativum - chemistry, Pisum sativum - enzymology, Plant Proteins - antagonists & inhibitors, Plant Proteins - chemistry, Plant Proteins - metabolism, Protein Binding - drug effects, Thylakoids - chemistry, Thylakoids - enzymology