Details

Autor(en) / Beteiligte

• Klug, Gabriele
• Kaufmann, Norbert
• Drews, Gerhart

Titel

Gene Expression of Pigment-Binding Proteins of the Bacterial Photosynthetic Apparatus: Transcription and Assembly in the Membrane of Rhodopseudomonas capsulata

Ist Teil von

• Proceedings of the National Academy of Sciences - PNAS, 1985-10, Vol.82 (19), p.6485-6489

Ort / Verlag

Washington, DC: National Academy of Sciences of the United States of America

Erscheinungsjahr

1985

Link zum Volltext

Quelle

EZB Free E-Journals

Beschreibungen/Notizen

• Lowering of oxygen partial pressure in chemotrophic cultures or reduction of light intensity in phototrophic cultures of Rhodopseudomonas capsulata induced formation of the photosynthetic apparatus. A maximum of mRNA coding for the reaction center (RC) and the light-harvesting I B870 antenna complex polypeptides occurred 30 min after induction. Maximal expression of mRNA for B800-B850 antenna proteins appeared with a lag time of about 25 min after RC/B870 mRNA. Pigment-binding polypeptides were inserted into the membrane immediately after mRNA synthesis. It is concluded that the delayed formation of the B800-B850 complex compared to the RC and the B870 complex is caused by sequential expression of the corresponding genes. Biological activity of pigment-protein complexes increased after the incorporation of their polypeptides parallel to the maximum of bacteriochlorophyll synthesis. Studies on mutant strains defective in the formation of pigment-protein complexes suggested that pigment synthesis is of importance for assembly of stable complexes.