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Distribution of STR locus DXS8027 polymorphism in the Han population in Qinba mountain areas
Ist Teil von
Yíchuán, 2006-03, Vol.28 (3), p.273
Ort / Verlag
China
Erscheinungsjahr
2006
Link zum Volltext
Quelle
MEDLINE
Beschreibungen/Notizen
To investigate the genetic polymorphism at DXS8027 STR locus for Han population in Qinba Mountain Areas and construct a preliminary population genetics database for this locus. We collected 550 venous blood samples from unrelated Han individuals of the Qinba Mountain Areas, Blood was treated with anticoagulant EDTA, and genomic DNA was obtained by phenol-chloroform extraction. Target fragments for the DXS8027 were amplified by PCR, separated on 8% non-denaturing polyacrylamide gel. electrophoresis and stained with 0.1% silver nitrate (AgNO3). Nine alleles were observed among the total samples, and the allele frequencies were in accordance with Hardy-Weinberg equilibrium (P>0.05) with relatively high heterozygosity (Het=0.7968). The results showed that there was a significant difference in the distribution of DXS8027 allele frequency between males and females (Chi 2=30.242, P<0.01), but the difference was not significant in the same gender groups of the two areas (Chi 2=4.703, P >0.05; Chi 2=14.952, P >0.05),