Zhonghua yi xue yi chuan xue za zhi, 2006-02, Vol.23 (1), p.37
2006
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Details
- Autor(en) / Beteiligte
- Titel
- The down regulation of VEGF gene expression may link to change the expression profile of genes in leukemia cell line K562
- Ist Teil von
- Zhonghua yi xue yi chuan xue za zhi, 2006-02, Vol.23 (1), p.37
- Ort / Verlag
- China
- Erscheinungsjahr
- 2006
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- To explore the potential effects of anti-VEGF hairpin ribozyme gene to gene expression profiles in leukemia cell line K562. The lipofectamine mediation was used to transfect the recombinant eukaryotic expression plasmid (pcDNA3-RZ) containing anti-VEGF hairpin ribozyme gene and the non-recombinant vector as control into K562 cells. And the positive clones were screened by G418. Ribozyme gene in K562 cells was confirmed by PCR. Fluorescent real time reverse transcription-PCR(RT-PCR) and Western blotting were employed to detect the expression of VEGF mRNA and protein in leukemia cells. cDNA microarray was used to explore the alteration of gene expression profiles when decreasing VEGF gene expression in leukemia cells. Expression of PCNA and GSN genes were verified by semi-quantitative RT-PCR. The pcDNA3-RZ and pcDNA3 had been transfected into the human leukemia cell line K562 and positive clones been screened by G418. Stable expression of the ribozyme gene in K562 cells was confirmed by PCR. The level of VEGF m
- Sprache
- Chinesisch
- Identifikatoren
- ISSN: 1003-9406Titel-ID: cdi_pubmed_primary_16456783
- Format
- –
- Schlagworte
- Down-Regulation - drug effects, Gene Expression - drug effects, Gene Expression Profiling, Humans, K562 Cells, Leukemia - genetics, Leukemia - metabolism, Leukemia - pathology, RNA, Catalytic - pharmacology, Vascular Endothelial Growth Factor A - antagonists & inhibitors, Vascular Endothelial Growth Factor A - genetics, Vascular Endothelial Growth Factor A - metabolism