Details

Autor(en) / Beteiligte

• Lavigne, Rob
• Noben, Jean-Paul
• Hertveldt, Kirsten
• Ceyssens, Pieter-Jan
• Briers, Yves
• Dumont, Debora
• Roucourt, Bart
• Krylov, Victor N
• Mesyanzhinov, Vadim V
• Robben, Johan
• Volckaert, Guido

Titel

The structural proteome of Pseudomonas aeruginosa bacteriophage phiKMV

Ist Teil von

• Microbiology (Society for General Microbiology), 2006-02, Vol.152 (Pt 2), p.529

Ort / Verlag

England

Erscheinungsjahr

2006

Quelle

MEDLINE

Beschreibungen/Notizen

• The structural proteome of phiKMV, a lytic bacteriophage infecting Pseudomonas aeruginosa, was analysed using two approaches. In one approach, structural proteins of the phage were fractionated by SDS-PAGE for identification by liquid chromatography-mass spectrometry (LC-MS). In a second approach, a whole-phage shotgun analysis (WSA) was applied. WSA uses trypsin digestion of whole phage particles, followed by reversed-phase HPLC and gas-phase fractionation of the complex peptide mixture prior to MS. The results yield a comprehensive view of structure-related proteins in phiKMV and suggest subtle structural differences from phage T7.