Details

Autor(en) / Beteiligte

• Hou, Li
• Zhao, Yue-ran
• Wang, Lai-cheng
• Jiao, Yu-lian
• Zhang, Jie
• Ma, Chun-yan
• Cui, Bin

Titel

Construction of co-expression vector pSLC-IRES-IL-2 and its expression in COS-7 cells

Ist Teil von

• Xi bao yu fen zi mian yi xue za zhi, 2005-09, Vol.21 (5), p.561

Ort / Verlag

China

Erscheinungsjahr

2005

Quelle

MEDLINE

Beschreibungen/Notizen

• To construct an eukaryotic co-expression plasmid pSLC-IRES-IL-2, and to express it in COS-7 cells. Human IL-2 and SLC genes were cloned by RT-PCR and PCR, respectively, and then the eukaryotic expression plasmid pSLC-IRES-IL-2 was constructed.The constructed plasmid was transfected into COS-7 cells by electroporation method. The expression of SLC and IL-2 was detected by Western blot. SLC and IL-2 in the culture supernatant and lysate of transfected COS-7 cells were detected by Western blot. The relative molecular masses of the expressed products were consistent with the theoretical values. The SLC and IL-2 co-expression plasmid is successfully constructed and expressed in COS-7 cells, which offers a pathway for research on gene therapy of tumors.