Details

Autor(en) / Beteiligte

• Park, Hae-Jeong
• Yoon, Seo-Hyun
• Han, Long-Shan
• Zheng, Long-Tai
• Jung, Kyung-Hee
• Uhm, Yoon-Kyung
• Lee, Je-Hyun
• Jeong, Ji-Seon
• Joo, Woo-Sang
• Yim, Sung-Vin
• Chung, Joo-Ho
• Hong, Seon-Pyo

Titel

Amygdalin inhibits genes related to cell cycle in SNU-C4 human colon cancer cells

Ist Teil von

• World journal of gastroenterology : WJG, 2005-09, Vol.11 (33), p.5156

Ort / Verlag

United States

Erscheinungsjahr

2005

Quelle

MEDLINE

Beschreibungen/Notizen

• The genes were divided into seven categories according to biological function; apoptosis-related, immune response-related, signal transduction-related, cell cycle-related, cell growth-related, stress response-related and transcription-related genes. We compared the gene expression profiles of SNU-C4 cells between amygdalin-treated (5 mg/mL, 24 h) and non-treated groups using cDNA microarray analysis. We selected genes downregulated in cDNA microarray and investigated mRNA levels of the genes by RT-PCR. Microarray showed that amygdalin downregulated especially genes belonging to cell cycle category: exonuclease 1 (EXO1), ATP-binding cassette, sub-family F, member 2 (ABCF2), MRE11 meiotic recombination 11 homolog A (MRE11A), topoisomerase (DNA) I (TOP1), and FK506 binding protein 12-rapamycin-associated protein 1 (FRAP1). RT-PCR analysis revealed that mRNA levels of these genes were also decreased by amygdalin treatment in SNU-C4 human colon cancer cells. These results suggest that amygdalin have an anticancer effect via downregulation of cell cycle-related genes in SNU-C4 human colon cancer cells, and might be used for therapeutic anticancer drug.