Zhongguo zhongyao zazhi, 2005-06, Vol.30 (11), p.851
2005
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- Autor(en) / Beteiligte
- Titel
- Purification and characterization of the proliferation of rat osteoblast-like cells UMR-106 from pilose antler
- Ist Teil von
- Zhongguo zhongyao zazhi, 2005-06, Vol.30 (11), p.851
- Ort / Verlag
- China
- Erscheinungsjahr
- 2005
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- The activity of deer serum albumin on proliferation of rat osteogenic-like cells UMR-106 and the IGF-I secretion were investigated in order to elucidate pilose antler's bone-strengthening mechanism. Deer serum albumin was isolated from freeze-dry pilose antler powder extract. The methods were Sephacryl S-200HR gel filtration, POROS 20QE ion-exchange and TSK G3000SW chromatographies. The effect of deer serum albumin on proliferatio of UMR-106 cells was assaied by MTT, and the secretion of IGF-I of UMR-106 cells was assaied by RIA. Deer serum albumin, with the molecular weight of 56.3 kDa, significantly increased the proliferation rate of the osteoblast-like UMR-106 cell and IGF-I secretion. When concentration of deer serum albumin reached 0.149 microg x mL(-1), UMR-106 cell proliferation rate was 241.03% and IGF-I secretion was 66.89 ng x mL(-1). The concentration of deer serum albumin, from 14.9 ng x mL(-1) to 14.90 microg x mL(-1), significantly increased the proliferation rate of the osteoblast-like UMR-106
- Sprache
- Chinesisch
- Identifikatoren
- ISSN: 1001-5302Titel-ID: cdi_pubmed_primary_16110870
- Format
- –
- Schlagworte
- Animals, Antlers - chemistry, Bone Neoplasms - pathology, Cell Line, Tumor, Cell Proliferation - drug effects, Deer, Insulin-Like Growth Factor I - secretion, Materia Medica - isolation & purification, Materia Medica - pharmacology, Osteoblasts - metabolism, Osteoblasts - pathology, Osteosarcoma - pathology, Rats, Serum Albumin - isolation & purification, Serum Albumin - pharmacology