Free radical research, 2005-09, Vol.39 (9), p.995-1003
2005
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Details
- Autor(en) / Beteiligte
- Titel
- Rosmarinic acid inhibits the formation of reactive oxygen and nitrogen species in RAW264.7 macrophages
- Ist Teil von
- Free radical research, 2005-09, Vol.39 (9), p.995-1003
- Ort / Verlag
- England: Informa UK Ltd
- Erscheinungsjahr
- 2005
- Quelle
- Taylor & Francis
- Beschreibungen/Notizen
- Antioxidant action of Rosmarinic acid (Ros A), a natural phenolic ingredient in many Lamiaceae herbs such as Perilla frutescens, sage, basil and mint, was analyzed in relation to the Iκ-B activation in RAW264.7 macrophages. Ros A inhibited nitric oxide (NO) production and inducible nitric oxide synthase (iNOS) protein synthesis induced by lipopolysaccharide (LPS), and also effectively suppressed phorbol 12-myristate 13-acetate (PMA)-induced superoxide production in RAW264.7 macrophages in a dose-dependent manner. Peroxynitrite-induced formation of 3-nitrotyrosine in bovine serum albumin and RAW264.7 macrophages were also inhibited by Ros A. Moreover, Western blot analysis demonstrated that LPS-induced phosphorylation of Iκ-Bα was abolished by Ros A. Ros A can act as an effective protector against peroxynitrite-mediated damage, and as a potent inhibitor of superoxide and NO synthesis; the inhibition of the formation of reactive oxygen and nitrogen species are partly based on its ability to inhibit the serine phosphorylation of Iκ-Bα.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1071-5762eISSN: 1029-2470DOI: 10.1080/10715760500231836Titel-ID: cdi_pubmed_primary_16087481
- Format
- –
- Schlagworte
- Animals, Antioxidants - pharmacology, Cell Line, Cell Survival, Cinnamates - pharmacology, Depsides, Dose-Response Relationship, Drug, I-kappa B Proteins - metabolism, Iκ-Bα, Lipopolysaccharides, Macrophages - drug effects, Macrophages - metabolism, Mice, nitric oxide, Nitric Oxide - metabolism, peroxynitrite, Phosphorylation - drug effects, RAW264.7 macrophages, Reactive Nitrogen Species - antagonists & inhibitors, Reactive Nitrogen Species - metabolism, Reactive Oxygen Species - antagonists & inhibitors, Reactive Oxygen Species - metabolism, Rosmarinic Acid, Serine - metabolism, superoxide, Superoxides - metabolism, Tetradecanoylphorbol Acetate - analogs & derivatives, Tyrosine - metabolism