Details

Autor(en) / Beteiligte

• Sun, Yong-xin
• Zhao, Qiang
• Wang, Yi-qing
• Yang, Cheng
• Pan, Cui-zhen
• Han, Pei-pei
• Chen, Rui-zhen
• Yang, Ying-zhen
• Wang, Ke-qiang
• Ge, Jun-bo

Titel

Upregulating the expression of angiogenesis-related genes by transplanting autologous mononuclear bone marrow cells into myocardial infarction scar and the periphery

Ist Teil von

• Zhōnghuá xīnxuèguănbìng zázhì, 2005-03, Vol.33 (3), p.260

Ort / Verlag

China

Erscheinungsjahr

2005

Quelle

MEDLINE

Beschreibungen/Notizen

• To detect the regulation of angiogenic genes involved in the processes of collateral development. Myocardial infarction (MI) scar was induced by cryoinjury in New Zealand rabbits. Four weeks after MI, 24 hours before cell transplantation, bone marrow was aspirated from the right thigh bone and mononuclear bone marrow cells (BMCs) were isolated by Ficoll density gradient centrifugation. Then the mononuclear BMCs (n = 8) or IMDM culture medium (n = 8) were transplanted into infarction scar and the periphery. Four weeks after mononuclear BMCs transplantation, DNA microarray analysis was performed to detect the regulation of angiogenesis-related genes in infarction scar and the periphery. And the differences of angiogenic genes expression were compared among several important growth factors by Western blot. DNA microarray analysis showed the detail regulation of genes involved in the angiogenic processes. There were 15 genes upregulated over 3 times in the infarction scar. In addition, we also found more genes ar