Details

Autor(en) / Beteiligte

• Wu, Qiaofeng
• Tang, Yanjuan
• Chen, Huaiqing
• Wu, Jiang
• Yin, Guangfu

Titel

Research of osteoblast-induced rat mesenchymal stem cells cocultured with beta-TCP/PLLA composite of different ratio

Ist Teil von

• Sheng wu yi xue gong cheng xue za zhi, 2005-04, Vol.22 (2), p.242

Ort / Verlag

China

Erscheinungsjahr

2005

Quelle

MEDLINE

Beschreibungen/Notizen

• There are three key factors in tissue engineering: seeding cells, scaffold and their interaction. Although mesenchymal stem cells (MSCs) are potential seeding cells, the problem of what phase MSCs should be used is not yet solved. On the other hand, degradable porous scaffolds which have good mechanics and good biocompatibility are preferred. To choose the optimum seeding cells and the suitable ratio of beta-TCP/PLLA porous scaffold, we observed the phenotype of the male SD rat's osteoblastic MSCs and detected the amount of alkaline phosphatase, osteocalcin and type I collagen secreted by the osteoblastic rMSCs in different phase. About 10, 14 and 20 days after induction, the induced cells came into proliferative phase, matrix synthesis phase and mineralization phase, respectively. Then we chose the suitable cells and seeded them on beta-TCP/PLLA composite scaffolds with different ratios (beta-TCP/PLLA = 1:1; beta-TCP/PLLA = 1:2; and beta-TCP/PLLA = 2:1). Fluorescence microscope, scanning electron microscope