Details

Autor(en) / Beteiligte

• Cao, Guo-qiang
• Qian, Gui-sheng
• Lin, Li
• Cui, She-huai

Titel

The changes of apoptosis and proliferation of pulmonary tissue in chronic obstructive pulmonary disease rats

Ist Teil von

• Zhonghua jiehe he huxi zazhi, 2005-03, Vol.28 (3), p.169

Ort / Verlag

China

Erscheinungsjahr

2005

Quelle

MEDLINE

Beschreibungen/Notizen

• To observe the changes of apoptosis and proliferation of pulmonary tissue cells in rats with chronic obstructive pulmonary disease (COPD). The rat model of COPD was established by exposure to cigarette smoking. Thirty-three Wistar rats were randomly divided into a normal control (NC) group, a smoking inhalation for 1 month (COPD-1) group and a smoking inhalation for 2 month (COPD-2) group. Pathologic changes of lung tissues and inflammatory cell differentials were studied. Immunohistochemistry and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) methods were carried out to examine the percentage of positive cells and distribution of apoptotic cells and proliferating cells in the lung tissue. Significant increases in total leukocyte numbers and neutrophils in the bronchoalveolar lavage were found in the COPD groups as compared to NC group. Two months after smoking exposure, enlargement of airspaces distal to the terminal bronchiole, destruction of alveolar walls, and loss of the al