Ai zheng = Aizheng = Chinese journal of cancer, 2005-01, Vol.24 (1), p.23
2005
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- Autor(en) / Beteiligte
- Titel
- Effects of chemokine receptor and its ligand on migration of ovarian cancer cells
- Ist Teil von
- Ai zheng = Aizheng = Chinese journal of cancer, 2005-01, Vol.24 (1), p.23
- Ort / Verlag
- China
- Erscheinungsjahr
- 2005
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Chemokine receptors express on many tumor cells, and closely correlate with migration and metastasis of tumor cells. This study was to investigate expressions of chemokine(C-X-C) receptor 4 (CXCR4) and chemokine (C-X-C motif) ligand 12 (CXCL12) in human ovarian epithelial tumor cells, and their effects on migration of tumor cells. Expression of CXCR4 mRNA and protein in 15 specimens of epithelial ovarian cancer tissue, ovarian cancer cell line CAOV3, endothelial cell line HUVEC, and 10 specimens of normal ovary tissue were evaluated by reverse transcriptase-polymerase chain reaction (RT-PCR) and Western blot. Expression of CXCL12 mRNA in retroperitoneal lymph nodes, and smooth muscle of fallopian tube from the same 15 epithelial ovarian cancer patients was tested by RT-PCR, quantity of CXCL12 in ascites of 15 patients was assayed using ELISA. Boyden Transwells was used to analyze effects of CXCL12, and cancerous ascites on chemotaxis of CAOV3, and HUVEC cells. (1) Expression levels of CXCR4 mRNA in ovary canc
- Sprache
- Chinesisch
- Identifikatoren
- Titel-ID: cdi_pubmed_primary_15642195
- Format
- –
- Schlagworte
- Adult, Aged, Ascitic Fluid - metabolism, Cell Line, Tumor, Cell Movement, Chemokine CXCL12, Chemokines, CXC - biosynthesis, Chemokines, CXC - genetics, Chemokines, CXC - physiology, Chemotaxis, Epithelial Cells - metabolism, Female, Humans, Lymph Nodes - metabolism, Middle Aged, Neoplasm Metastasis, Ovarian Neoplasms - metabolism, Ovarian Neoplasms - pathology, Receptors, CXCR4 - biosynthesis, Receptors, CXCR4 - genetics, Receptors, CXCR4 - physiology, RNA, Messenger - biosynthesis, RNA, Messenger - genetics