Journal of Clinical Microbiology, 2005, Vol.43 (1), p.150-155
2005
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- Autor(en) / Beteiligte
- Titel
- Comparison of emm Typing and Ribotyping with Three Restriction Enzymes To Characterize Clinical Isolates of Streptococcus pyogenes
- Ist Teil von
- Journal of Clinical Microbiology, 2005, Vol.43 (1), p.150-155
- Ort / Verlag
- Washington, DC: American Society for Microbiology
- Erscheinungsjahr
- 2005
- Quelle
- Free E-Journal (出版社公開部分のみ）
- Beschreibungen/Notizen
- A total of 336 Streptococcus pyogenes isolates recently recovered from patients with pharyngitis from 13 countries were characterized by emm typing and riboprinting using an automated Riboprinter (Dupont/Qualicon) based on the patterns produced by three restriction enzymes, EcoRI, PstI, and HindIII. Three enzymes were necessary to increase the discrimination of ribogroups formed by each enzyme. A total of 40 ribogroups and 38 emm sequences (not counting allelic variations) were identified. Multilocus sequence typing was performed on a sampling of the isolates, and those results were consistent with those of both emm typing and ribotyping. Correlations were observed among all three methods.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0095-1137eISSN: 1098-660XDOI: 10.1128/JCM.43.1.150-155.2005Titel-ID: cdi_pubmed_primary_15634964
- Format
- –
- Schlagworte
- Antigens, Bacterial - genetics, Antigens, Bacterial - metabolism, Bacterial Outer Membrane Proteins - genetics, Bacterial Outer Membrane Proteins - metabolism, Bacterial Typing Techniques, Bacteriology, Biological and medical sciences, Carrier Proteins - genetics, Carrier Proteins - metabolism, Deoxyribonuclease EcoRI - metabolism, Deoxyribonuclease HindIII - metabolism, Deoxyribonucleases, Type II Site-Specific - metabolism, Fundamental and applied biological sciences. Psychology, Humans, Infectious diseases, Medical sciences, Microbiology, Miscellaneous, Pharyngitis - microbiology, Ribotyping, Streptococcal Infections - microbiology, Streptococcus pyogenes, Streptococcus pyogenes - classification, Streptococcus pyogenes - genetics, Streptococcus pyogenes - isolation & purification