Details

Autor(en) / Beteiligte

• Silwood, Christopher J.L.
• Chikanza, Ian C.
• Tanner, K. Elizabeth
• Shelton, Julia C.
• Bowsher, John G.
• Grootveld, Martin

Titel

Investigation of the Molecular Nature of Low-molecular-mass Cobalt(II) Ions in Isolated Osteoarthritic Knee-joint Synovial Fluid

Ist Teil von

• Free radical research, 2004-06, Vol.38 (6), p.561-571

Ort / Verlag

England: Informa UK Ltd

Erscheinungsjahr

2004

Quelle

Taylor & Francis Journals Auto-Holdings Collection

Beschreibungen/Notizen

• High field 1H NMR spectroscopy demonstrated that addition of Co(II) ions to osteoarthritic knee-joint synovial fluid (SF) resulted in its complexation by a range of biomolecules, the relative efficacies of these complexants/chelators being citrate > histidine ∼ threonine>glycine ∼ glutamate ∼ glutamine ∼ phenylalanine ∼ tyrosine > formate > lactate>alanine > valine > acetate > pyruvate > creatinine, this order reflecting the ability of these ligands to compete for the available Co(II) in terms of (1) thermodynamic equilibrium constants for the formation of their complexes and (2) their SF concentrations. Since many of these SF Co(II) complexants (e.g. histidinate) serve as powerful *OH scavengers, the results acquired indicate that any of this radical generated from the Co(II) source in such complexes via Fenton or pseudo-Fenton reaction systems will be "site-specifically" scavenged. The significance of these observations with regard to cobalt toxicity and the in vivo corrosion of cobalt-containing metal alloy joint prostheses (e.g. CoCr alloys) is discussed.