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Interleukin-8 induces neutrophil accumulation but not protease secretion in the canine trachea
Ist Teil von
American journal of physiology. Lung cellular and molecular physiology, 1992-12, Vol.263 (6), p.708-L713
Ort / Verlag
United States
Erscheinungsjahr
1992
Quelle
MEDLINE
Beschreibungen/Notizen
P. G. Jorens, J. B. Richman-Eisenstat, B. P. Housset, P. D. Graf, I. F. Ueki, J. Olesch and J. A. Nadel
Cardiovascular Research Institute, University of California, San Francisco 94143-0130.
The neutrophil enzyme elastase is a potent secretagogue of airway secretory
cells, and elastase is present in high concentrations in sputum of patients
with hypersecretion (e.g., cystic fibrosis, bronchiectasis). Interleukin-8
(IL-8), a recently discovered cytokine with potent neutrophil chemotactic
properties in vitro, is also found in the sputum of these patients. We used
an isolated tracheal segment in dogs in vivo to study the effect of IL-8 in
causing neutrophil accumulation, elastase release, and secretion (by
measuring lysozyme concentrations) in the luminal superfusate. IL-8 caused
a potent time-dependent neutrophil accumulation at between 3 and 6 h. The
effect was significant at 10(-9) and maximum at 10(-8) M. No increase in
free elastase, cathepsin G, or lysozyme was detected in the superfusate.
Thus, in contrast to previous studies showing that ragweed antigen causes
the accumulation of neutrophil elastase which in turn causes lysozyme
secretion, IL-8 causes neutrophil accumulation without granule secretion
(or subsequent secretagogue activity). The findings were confirmed with dog
and human neutrophils in vitro.