Details

Autor(en) / Beteiligte

• Konishi, Y. (Kirin Brewery Co. Ltd., Takasaki, Gunma (Japan). Applied Bioresearch Center)
• Kobayashi, S
• Shimizu, M

Titel

Transepithelial transport of p-coumaric acid and gallic acid in caco-2 cell monolayers

Ist Teil von

• Bioscience, biotechnology, and biochemistry, 2003-11, Vol.67 (11), p.2317-2324

Ort / Verlag

Tokyo: Japan Society for Bioscience, Biotechnology, and Agrochemistry

Erscheinungsjahr

2003

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• The transepithelial transport of such common dietary phenolic acids as p-coumaric acid (CA) and gallic acid (GA) across Caco-2 cell monolayers was examined. CA transport was dependent on pH, and in a vectorial manner in the apical-basolateral direction. The permeation was concentration-dependent and saturable, the Michaelis constant and maximum velocity being 17.5 mm and 82.7 nmol min sup (-1) (mg of protein) sup (-1) respectively. Benzoic acid and acetic acid inhibited the permeation of CA. These results indicate that the transepithelial transport of CA was via the monocarboxylic acid transporter (MCT). On the other hand, the permeation of GA was not in a polarized manner, was independent of pH and linearly increased with increasing concentration of GA. The transport rate of GA was about 100 times lower than that of CA, suggesting the transepithelial transport of GA to be via the paracellular pathway. Dietary phenolic acids thus showed diversified characteristics in their intestinal absorption.