Details

Autor(en) / Beteiligte

• e Silva, P M
• Martins, M A
• Lima, M C
• Alves, A C
• Diaz, B L
• Cordeiro, R S

Titel

Pharmacological modulation of the late eosinophilia induced by antigen in actively sensitized rats

Ist Teil von

• International archives of allergy and immunology, 1992-01, Vol.98 (4), p.355-360

Ort / Verlag

Switzerland

Erscheinungsjahr

1992

Quelle

MEDLINE

Beschreibungen/Notizen

• The intrathoracic injection of ovalbumin (12 micrograms/cavity) into actively sensitized rats led to a long-lasting eosinophil recruitment, which appeared 24 h after stimulation. In this study, pharmacological antagonists were used in order to evaluate the potential involvement of arachidonic acid metabolites and PAF-acether in the pleural eosinophil accumulation by antigen. Administration of the cyclooxygenase inhibitor indomethacin (2 mg/kg, i.p.), 1 h before the antigen challenge, failed to modify the 24-hour eosinophilia. In contrast, the dual cyclooxygenase and lipoxygenase inhibitor BW 755C and the more selective inhibitor BW A4C (5 and 10 micrograms/cavity, i.t.), injected 1 h before the antigen, were effective. Similarly, the PAF-acether antagonists BN 52021 and WEB 2086 (20 mg/kg, i.p.) abrogated the eosinophil accumulation, which was also sensitive to the topical treatment with the glucocorticoid dexamethasone (5 and 10 micrograms/cavity). Our findings suggest that the antigen-induced eosinophil mobilization is dependent on lipoxygenase derivatives and PAF-acether, but not on prostaglandins.