Details

Autor(en) / Beteiligte

• Korver, T H
• van de Stadt, R J
• Kiljan, E
• van Kampen, G P
• van der Korst, J K

Titel

Effects of loading on the synthesis of proteoglycans in different layers of anatomically intact articular cartilage in vitro

Ist Teil von

• Journal of rheumatology, 1992-06, Vol.19 (6), p.905

Ort / Verlag

Canada

Erscheinungsjahr

1992

Quelle

MEDLINE

Beschreibungen/Notizen

• The anatomically intact articular cartilage (approximately equal to 2.5 cm2) of whole bovine sesamoid bones was cultured on its bone support. A load of 5 kg was applied intermittently at 0.3 Hz by a specially designed loading apparatus for 7 days. [35S]-sulfate was added during the last 17 h of the experiment. Loading induced a 40% increase in [35S]-sulfate incorporation into aggrecans and an almost 3-fold increase in the synthesis of small proteoglycans. The loading effects occurred mainly in the upper half of the articular cartilage. Samples left unloaded for 7 days and then loaded for 7 days likewise showed an increase in [35S]-sulfate incorporation compared with unloaded controls.