Details

Autor(en) / Beteiligte

• COOK, F S

Titel

Adaptation of the carbon dioxide yield in the ninhydrin reaction to the study of transamination in plant tissues

Ist Teil von

• Canadian journal of biochemistry and physiology, 1956-01, Vol.34 (1), p.15-24

Ort / Verlag

Canada

Erscheinungsjahr

1956

Quelle

MEDLINE

Beschreibungen/Notizen

• A simple technique for assaying the glutamic–aspartic transaminase activity in plant tissues making use of titrimetric measurements of the carbon dioxide yield in the ninhydrin reaction with alpha-amino acids is described. The necessary apparatus is simple and inexpensive. The success of this method is verified by comparing results with those obtained on the same or similar reaction mixtures by quantitative paper chromatography, and by the chloramine-T method. Kinetic data on enzyme preparations of corn radicle homogenates and radicle segments frozen intact have been successfully obtained. The glutamic–aspartic transaminase is very active in corn radicles, giving high initial velocities. Per cent transamination per 10 min. period varies linearly with enzyme concentration. The optimum pH for the homogenates is at 8.5.