Details

Autor(en) / Beteiligte

• Maréchal, Joëlle
• Santos, Renata
• Hammad, Yasser
• Alloisio, Nicole
• Domenach, Anne-Marie
• Normand, Philippe

Titel

Characterization of the sodF gene region of Frankia sp. strain ACN14a and complementation of Escherichia coli sod mutant

Ist Teil von

• Canadian journal of microbiology, 2003-04, Vol.49 (4), p.294-300

Ort / Verlag

Canada

Erscheinungsjahr

2003

Quelle

MEDLINE

Beschreibungen/Notizen

• The Frankia sp. strain ACN14a superoxide dismutase SodF was previously shown to be induced in response to Alnus glutinosa root exudates, and its gene was sequenced. We report here the sequence of the 9-kb genomic segment surrounding the sodF gene and further characterize this gene and its product. Nine ORFs coding for various proteins, such as regulators, acetyl-CoA transferases, and a bacterioferritin A next to the sodF gene, were found. Northern blot analysis showed that the sodF gene was expressed as a major 1-kb transcript, which indicates that it has its own promoter. The sodF gene strongly complemented an Escherichia coli triple mutant (sodA sodB recA), restoring aerobic growth when the gene was expressed from the synthetic tac promoter but when expressed from its own promoter showed only slight rescue, suggesting that it was poorly recognized by the E. coli RNA polymerase. It is noteworthy that this is the first time that a Frankia gene has been reported to complement an E. coli mutant. The superoxide dismutase activity of the protein was inactivated by hydrogen peroxide, indicating that the metal ligand is iron, which is supported by analysis of the protein sequence. Thus, the SodF protein induced in Frankia by root exudates is an iron-containing enzyme similar to the one present in the nodules.