Details

Autor(en) / Beteiligte

• Xu, Yong
• Zhang, Ni
• Huang, Songmin
• Fu, Ping
• Liu, Xiaojing
• Zhang, Haiyang
• Qiang, Ou

Titel

Culture of mesangial cells in two different states and a study on their functions

Ist Teil von

• Hua xi yi ke da xue xue bao = Journal of West China University of Medical Sciences = Huaxi yike daxue xuebao, 2002-04, Vol.33 (2), p.223

Ort / Verlag

China

Erscheinungsjahr

2002

Quelle

MEDLINE

Beschreibungen/Notizen

• To make a comparision of cell function between the mesangial cells from rats with diabetic mellitus (DMC) and the mesangial cells stimulated by high glucose (HMC) and to explore the feasibility and advantage of culturing mesangial cells (MC) from diabetic rats in studies on the pathogenesis of diabetic nephropathy. DMC were obtained from rats with streptozotocin-induced diabetes and were cultivated in normal medium. The MC from normal rats were divided into HMC high glucose and NMC cultured in normal medium as control. The cellular proliferation was assessed using 3H-thymidine incorporation. Production of fibronectin (FN) was analysed by ELISA. And with the use of laser scanning confocal microscopy, the calcium concentration ([Ca2+]i) and the response of [Ca2+]i to Angiotensin II (Ang II) were measured. The 3H-TdR incorporation efficiency of DMC was significantly higher than that of CMC; the 3H-TdR incorporation efficiency of HMC was lower than that of CMC (P < 0.05). The secretion of FN in DMC and HMC was hi