Zhongguo shi yan xue ye xue za zhi, 2002-02, Vol.10 (1), p.35
2002
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Details
- Autor(en) / Beteiligte
- Titel
- The expression of Fas, FasL and Bcl-2 on RMA cells during the process of apoptosis induced by chemotherapeutic drugs
- Ist Teil von
- Zhongguo shi yan xue ye xue za zhi, 2002-02, Vol.10 (1), p.35
- Ort / Verlag
- China
- Erscheinungsjahr
- 2002
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- The objective of the study is to explore the effect of Fas, FasL and Bcl-2 on the process of apoptosis induced by chemotherapeutic drugs through detecting the expression of Fas, FasL and Bcl-2 on murine lymphoma cell line RMA. Dexamethasone(DEX), etoposide (VP-16), arsenic trioxide As(2)O(3) and all trans-retinoic-acid (ATRA) were added to the RMA cells as well as to the cells preincubated with interleukin-2 (IL-2), interleukin-6 (IL-6) or granulocyte-macrophage colony-stimulating factor (GM-CSF), respectively. The effect on apoptosis was observed and the expression of Fas and FasL mRNA as well as the expression of Fas and Bcl-2 antigen were measured. DEX and VP-16 could promote apoptosis of RMA cells while upregulating the expression of Fas and FasL without affecting the expression of Bcl-2. ATRA downregulated the expression of Bcl-2 without any change of Fas and FasL, and no apoptosis of RMA cells induced by ATRA was observed. Although As(2)O(3) induced apoptosis of RMA cells, it did not affect the expressi
- Sprache
- Chinesisch
- Identifikatoren
- ISSN: 1009-2137Titel-ID: cdi_pubmed_primary_12513834
- Format
- –
- Schlagworte
- Animals, Antineoplastic Combined Chemotherapy Protocols - pharmacology, Apoptosis - physiology, Arsenicals - pharmacology, Dexamethasone - pharmacology, Etoposide - pharmacology, Fas Ligand Protein, fas Receptor - biosynthesis, Gene Expression - drug effects, Membrane Glycoproteins - biosynthesis, Mice, Oxides - pharmacology, Proto-Oncogene Proteins c-bcl-2 - biosynthesis, Tretinoin - pharmacology, Tumor Cells, Cultured