Details

Autor(en) / Beteiligte

• Wang, Xiaoxia
• Qin, Zheng‐Hong
• Leng, Yan
• Wang, Yumei
• Jin, Xiannu
• Chase, Thomas N.
• Bennett, M. Catherine

Titel

Prostaglandin A1 inhibits rotenone‐induced apoptosis in SH‐SY5Y cells

Ist Teil von

• Journal of neurochemistry, 2002-12, Vol.83 (5), p.1094-1102

Ort / Verlag

Oxford, UK: Blackwell Science Ltd

Erscheinungsjahr

2002

Quelle

MEDLINE

Beschreibungen/Notizen

• The degeneration of nigral dopamine neurons in Parkinson's disease (PD) reportedly involves a defect in brain mitochondrial complex I in association with the activation of nuclear factor‐κB (NF‐κB) and caspase‐3. To elucidate molecular mechanisms possibly linking these events, as well as to evaluate the neuroprotective potential of the cyclopentenone prostaglandin A1 (PGA1), an inducer of heat shock proteins (HSPs), we exposed human dopaminergic SH‐SY5Y cells to the complex I inhibitor rotenone. Dose‐dependent apoptosis was preceded by the nuclear translocation of NF‐κB and then the activation of caspase‐3 over the ensuing 24 h. PGA1 increased the expression of HSP70 and HSP27 and protected against rotenone‐induced apoptosis, without increasing necrotic death. PGA1 blocked the rotenone‐induced nuclear translocation of NF‐κB and attenuated, but did not abolish, the caspase‐3 elevation. Unexpectedly, the caspase‐3 inhibitor, Ac‐DEVD.CHO (DEVD), at a concentration that completely prevented the caspase‐3 elevation produced by rotenone, failed to protect against apoptosis. These results suggest that complex I deficiency in dopamine cells can induce apoptosis by a process involving early NF‐κB nuclear translocation and caspase‐3 activation. PGA1 appears to protect against rotenone‐induced cell death by inducing HSPs and blocking nuclear translocation of NF‐κB in a process that attenuates caspase‐3 activation, but is not mediated by its inhibition.