Details

Autor(en) / Beteiligte

• WOHLRAB, Johannes
• SIEMES, Christina
• MARSCH, Wolfgang C

Titel

The influence of L-arginine on the regulation of epidermal arginase

Ist Teil von

• Skin pharmacology and applied skin physiology, 2002, Vol.15 (1), p.44-54

Ort / Verlag

Basel: Karger

Erscheinungsjahr

2002

Quelle

MEDLINE

Beschreibungen/Notizen

• Topical preparations containing urea are firmly established in dermatological therapy and nursing care therapy. In addition, urea is frequently used as an inactive ingredient with disinfecting, keratoplastic and penetration-promoting action in topical preparations. Despite good tolerance and ensured action, particularly the irritating effect on erosive, exudative or strongly inflammatory skin restricts its application. Endogenic urea is synthesised from L-arginine by an extra-hepatic arginase in keratinocytes. This enzymatic reaction is subject to regulation by manganese ions and the intracellular L-arginine concentration. L-Arginine is predominantly transported into the cell by a membrane transport system that is dependent on the membrane potential. By incubating keratinocyte cultures in different concentrations of L-arginine and manganese chloride, it could be shown that the keratinocytic urea synthesis can be increased. In relevant concentrations, L-arginine and manganese chloride do not exhibit any proliferation-inhibiting action, do not trigger any apoptosis or necrosis, and are stable. An increase in the expression of arginase cannot be detected using L-arginine. The application of L-arginine alone or in combination with manganese chloride increases the endogenous intrakeratinocytic urea synthesis and thus offers an option for topical therapeutic application in cases of dry skin conditions.