International journal of oncology, 2001-02, Vol.18 (2), p.257
2001
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- Autor(en) / Beteiligte
- Titel
- Expression and function of interleukin-8 in human hepatocellular carcinoma
- Ist Teil von
- International journal of oncology, 2001-02, Vol.18 (2), p.257
- Ort / Verlag
- Greece
- Erscheinungsjahr
- 2001
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Expression and functions of interleukin (IL)-8, a pro-inflammatory cytokine with angiogenesis action, was examined in 23 surgically resected hepatocellular carcinoma (HCC) specimens and 7 HCC cell lines. In all HCC tissues, IL-8 expression was confirmed with reverse-transcription polymerase chain reaction method and enzyme-linked immunosorbent assay, and immunohistochemistry showed HCC cells were the major producer of IL-8 in the tissues. Microvessel density was measured by the double immunohistochemical staining of muscular vessels in HCC tissues, but the density was not related to the level of IL-8 in the HCC tissues. On the other hand, in the co-culture of human umbilical vein endothelial cells (HUVEC) and a HCC cell line (KIM-1), IL-8 produced by KIM-1 significantly accelerated the proliferation of HUVEC. In addition, cases with a high IL-8 level in cancerous tissue had a significantly higher frequency of portal vein invasion, venous invasion and bile duct invasion (p<0.05). In the cultures of 7 HCC cell lines IL-8 secretion into culture medium increased with the treatment of IL-1beta or tumor necrosis factor-alpha. This showed IL-8 expression is regulated by inflammatory cytokines. IL-8 produced by HCC is an angiogenesis factor of HCC, but it could have a much more important role in the invasion and metastasis of HCC.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1019-6439eISSN: 1791-2423DOI: 10.3892/ijo.18.2.257Titel-ID: cdi_pubmed_primary_11172590
- Format
- –
- Schlagworte
- Aged, Angiogenesis Inducing Agents - metabolism, Carcinoma, Hepatocellular - metabolism, Carrier Proteins - drug effects, Carrier Proteins - metabolism, Coculture Techniques, Cytokines - pharmacology, Endothelial Growth Factors - metabolism, Endothelium, Vascular - drug effects, Endothelium, Vascular - metabolism, Female, Humans, Intercellular Signaling Peptides and Proteins, Interleukin-8 - metabolism, Interleukin-8 - physiology, Liver Neoplasms - metabolism, Lymphokines - drug effects, Lymphokines - metabolism, Male, Middle Aged, Neoplasm Proteins - metabolism, Neoplasm Proteins - physiology, RNA, Messenger - drug effects, RNA, Messenger - metabolism, Tumor Cells, Cultured - drug effects, Tumor Cells, Cultured - metabolism, Vascular Endothelial Growth Factor A, Vascular Endothelial Growth Factors