Details

Autor(en) / Beteiligte

• Abramova, Z I
• Vinter, V G

Titel

Electron immunohistochemical analysis of localization of neutral Mn2+-dependent DNAase. II. Analysis of ultrastructural localization in epon sections of different organs of the rat

Ist Teil von

• T͡S︡itologii͡a, 2000, Vol.42 (7), p.688

Ort / Verlag

Russia (Federation)

Erscheinungsjahr

2000

Quelle

MEDLINE

Beschreibungen/Notizen

• The use of a conjugate of colloidal gold with monotypic antibodies against a neutral Mn(2+)-dependent DNAse has revealed the enzyme localization in ultrathin Epon sections of glutaraldehyde fixed tissues. Technical procedures involved in fixation, embedding, and immune reactions are described. DNAse has been established in the nuclei in both normal and regenerating liver. A protein, immunologically close to DNAse, has been identified in the nuclei of a lymph node, thymus, spleen and cerebellar cortex.