Details

Autor(en) / Beteiligte

• Akgur, Feza M
• Brown, Mark F
• Zibari, Gazi B
• McDonald, John C
• Epstein, Charles J
• Ross, Christopher R
• Granger, D. Neil

Titel

Role of superoxide in hemorrhagic shock-induced P-selectin expression

Ist Teil von

• American journal of physiology. Heart and circulatory physiology, 2000-08, Vol.279 (2), p.H791-H797

Ort / Verlag

United States

Erscheinungsjahr

2000

Quelle

MEDLINE

Beschreibungen/Notizen

• 1  Department of Pediatric Surgery, School of Medicine, Dokuz Eylül University, Izmir, Turkey 35340; Departments of 2  Surgery and 3  Molecular and Cellular Physiology, School of Medicine, Louisiana State University, Shreveport, Louisiana 71130; 4  Department of Pediatrics, University of California, San Francisco, California 94143; and 5  Department of Anatomy and Physiology, College of Veterinary Medicine, Kansas State University, Manhattan, Kansas 66506 Superoxide has been implicated in the regulation of endothelial cell adhesion molecule expression and the subsequent initiation of leukocyte-endothelial cell adhesion in different experimental models of inflammation. The objective of this study was to assess the contribution of oxygen radicals to P-selectin expression in a murine model of whole body ischemia-reperfusion, i.e., hemorrhage-resuscitation (H/R), with the use of different strategies that interfere with either the production (allopurinol, CD11/CD18-deficient or p47 phox / mice) or accumulation [intravenous superoxide dismutase (SOD), mutant mice that overexpress SOD] of oxygen radicals. P-selectin expression was quantified in different regional vascular beds by use of the dual-radiolabeled monoclonal antibody technique. H/R elicited a significant increase in P-selectin expression in all vascular beds. This response was blunted in SOD transgenic mice and in wild-type mice receiving either intravenous SOD or the xanthine oxidase inhibitor allopurinol. Mice genetically deficient in either a subunit of NADPH oxidase or the leukocyte adhesion molecule CD11/CD18 also exhibited a reduced P-selectin expression. These results implicate superoxide, derived from both xanthine oxidase and NADPH oxidase, as mediators of the increased P-selectin expression observed in different regional vascular beds exposed to hemorrhage and retransfusion. ischemia-reperfusion; leukocyte-endothelial cell adhesion; xanthine oxidase; 2 -integrins