Details

Autor(en) / Beteiligte

• Ghernati, I
• Corbin, A
• Chabanne, L
• Auger, C
• Magnol, J P
• Fournel, C
• Monier, J C
• Darlix, J L
• Rigal, D

Titel

Canine large granular lymphocyte leukemia and its derived cell line produce infectious retroviral particles

Ist Teil von

• Veterinary pathology, 2000-07, Vol.37 (4), p.310-317

Ort / Verlag

United States

Erscheinungsjahr

2000

Quelle

MEDLINE

Beschreibungen/Notizen

• We describe a case of large granular lymphocyte (LGL) leukemia in a dog that we followed over a period of 2 years. Analysis of a hematological profile revealed lymphocytosis (19,500 lymphocytes per microliter; reference values, 1,000-4,800 lymphocytes per microliter), with a majority of LGL on the blood smear. LGL is defined as a lymphoid subset comprising 10% of peripheral blood mononuclear cells and corresponding to either CD3- CD8- NK cells or CD3+ CD8+ T cells. The cells are characterized by abundant basophilic cytoplasm containing distinct granules of variable size and number. The characteristic phenotype of our leukemic LGL is of a cytotoxic T cell, CD3+ and CD8+. A new cell line, DLC 02, was established from the peripheral lymphocytes of the leukemic dog. Particles with type C retroviral morphology were found in ultrathin sections of DLC 02 cell pellets. These particles were found to have a sucrose gradient density of 1.17 g/liter and a reverse transcriptase activity with an Mn2+ preference, suggesting that they correspond to a mammalian type C oncovirus.