Details

Autor(en) / Beteiligte

• Massa, H M
• Bruce, N W

Titel

Effects of unilateral ovariectomy with compensatory hypertrophy on ovarian blood flow, oxygen consumption and progestin secretion in the 16-day pregnant rat

Ist Teil von

• Reproduction fertility and development, 1999, Vol.11 (3), p.153

Ort / Verlag

Australia

Erscheinungsjahr

1999

Quelle

MEDLINE

Beschreibungen/Notizen

• Stimulated ovulation with resultant multiple corpora lutea (CL) can result in lower progesterone levels than expected from the increased luteal tissue mass. Unilateral ovariectomy (ULO) was used to increase CL number in rats and determine whether this would compromise luteal tissue blood flow, oxygen consumption and progestin secretion. All investigations were performed in vivo, using a venous outflow technique on Day 16 of gestation, when progesterone secretion is maximal. ULO, performed before pregnancy, doubled CL number and total CL mass in the remaining ovary of six treated compared to five control rats. Growth of CL was not affected. The rate of ovarian blood flow (microL min(-1) mg CL(-1)) fell to 47% of control levels in ULO animals and progesterone secretion (microg h(-1) mg CL(-1)) to 68%. Secretion of the minor progestin, 20alpha-hydroxypregn-4-en-one was not affected. Tissue oxygen consumption was maintained despite the reduction in blood flow by an increase in oxygen extraction from arterial blood. These results suggest that overcrowding of CL in ULO-stimulated rat ovaries compromises luteal tissue blood flow and subsequently progesterone secretion.