Details

Autor(en) / Beteiligte

• Astrom, A
• D'Amore, E S
• Sainati, L
• Panarello, C
• Morerio, C
• Mark, J
• Stenman, G

Titel

Evidence of involvement of the PLAG1 gene in lipoblastomas

Ist Teil von

• International journal of oncology, 2000-06, Vol.16 (6), p.1107

Ort / Verlag

Greece

Erscheinungsjahr

2000

Quelle

MEDLINE

Beschreibungen/Notizen

• Previous cytogenetic studies have demonstrated that the majority of lipoblastomas show rearrangements, in particular translocations and insertions, with breakpoints in 8q11-13. Here we present evidence for involvement of the developmentally regulated zink finger gene PLAG1 in these rearrangements. Northern blot and RT-PCR analyses revealed overexpression of PLAG1 in two lipoblastomas. Using immunohistochemistry, expression of the PLAG1 protein was also demonstrated in tissue sections from two lipoblastomas, one of which had a t(3;8)(q13.1;q12) translocation and the other a t(1;6)(q42;p22) translocation. Since no aberrant PLAG1 transcripts could be detected, it is likely that the gene may be activated by promoter swapping/substitution or alternatively by an as yet unknown mechanism. Our findings indicate that PLAG1 activation is a recurrent event in lipoblastomas and that PLAG1 is likely to be the target gene on chromosome 8 in these tumors.