Details

Autor(en) / Beteiligte

• Pauza, C. David
• Trivedi, Parul
• Wallace, Marianne
• Ruckwardt, Tracy J.
• Le Buanec, Helene
• Lu, Wei
• Bizzini, Bernard
• Burny, Arsene
• Zagury, Daniel
• Gallo, Robert C.

Titel

Vaccination with Tat Toxoid Attenuates Disease in Simian/HIV-Challenged Macaques

Ist Teil von

• Proceedings of the National Academy of Sciences - PNAS, 2000-03, Vol.97 (7), p.3515-3519

Ort / Verlag

United States: National Academy of Sciences of the United States of America

Erscheinungsjahr

2000

Quelle

Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals

Beschreibungen/Notizen

• The Tat protein is essential for HIV type 1 (HIV-1) replication and may be an important virulence factor in vivo. We studied the role of Tat in viral pathogenesis by immunizing rhesus macaques with chemically inactivated Tat toxoid and challenging these animals by intrarectal inoculation with the simian/human immunodeficiency virus 89.6PD. Immune animals had significantly attenuated disease with lowered viral RNA, interferon-α and chemokine receptor expression (CXCR4 and CCR5) on CD4+T cells; these features of infection have been linked to in vitro effects of Tat and respond similarly to extracellular Tat protein produced during infection. Immunization with Tat toxoid inhibits key steps in viral pathogenesis and should be included in therapeutic or preventive HIV-1 vaccines.