Journal of endocrinological investigation, 2000-01, Vol.23 (1), p.1
2000
Volltextzugriff (PDF)
Details
- Autor(en) / Beteiligte
- Titel
- In vitro expression of proalpha1(I) collagen mRNA by human pre-osteoclastic cells
- Ist Teil von
- Journal of endocrinological investigation, 2000-01, Vol.23 (1), p.1
- Ort / Verlag
- Italy
- Erscheinungsjahr
- 2000
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- In vitro studies have demonstrated that the extracellular matrix modulates the cell phenotype. In the present study we have investigated in vitro proalpha1(I) collagen mRNA expression in a human pre-osteoclastic cell line (FLG 29.1 cells) in basal condition and after various stimuli. In addition, in order to evaluate the effect of cell-cell interactions on collagen type I mRNA expression, we have cultured the human pre-osteoclastic cells FLG 29.1 with either the human osteoblast-like cell line Saos-2 or the bovine bone endothelial cell line BBE. We showed that the FLG 29.1 cells express proal (I) collagen mRNA, whose expression is modulated by phorbol esters (TPA). Co-culturing FLG 29.1 cells with either Saos-2 or BBE cells induced decrease of proalpha1(I) collagen mRNA expression.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0391-4097eISSN: 1720-8386Titel-ID: cdi_pubmed_primary_10698043
- Format
- –
- Schlagworte
- Animals, Bone and Bones - metabolism, Cattle, Cell Communication, Coculture Techniques, Endothelium - metabolism, Gene Expression, Humans, Leukemia, Monocytic, Acute, Osteoblasts - metabolism, Osteoclasts - metabolism, Procollagen - genetics, RNA, Messenger - metabolism, Stem Cells - metabolism, Tumor Cells, Cultured