Details

Autor(en) / Beteiligte

• van den Berg, A
• van der Leij, J
• Poppema, S

Titel

Serial analysis of gene expression: rapid RT-PCR analysis of unknown SAGE tags

Ist Teil von

• Nucleic acids research, 1999-09, Vol.27 (17), p.e17

Ort / Verlag

England

Erscheinungsjahr

1999

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• In a pilot study on SAGE on Reed-Sternberg cells we have sequenced 1055 tags representing 701 genes. Screening of the GenBank database resulted in the identification of a corresponding gene or EST for 490 of them. For 211 of the tags no homology could be detected. A major problem of the serial analysis of gene expression (SAGE) approach is how to further analyse the unknown tags. We have developed an RT-PCR-based method, rapid analysis of unknown SAGE tags (RAST-PCR), to analyse the expression of the corresponding genes. This approach can be used as a screening method to investigate whether or not the gene is differentially expressed between several cell types of interest.