Details

Autor(en) / Beteiligte

• Gomes, Daniel G.
• Guimarães, Pedro M.R.
• Pereira, Francisco B.
• Teixeira, José A.
• Domingues, Lucília

Titel

Plasmid-mediate transfer of FLO1 into industrial Saccharomyces cerevisiae PE-2 strain creates a strain useful for repeat-batch fermentations involving flocculation–sedimentation

Ist Teil von

• Bioresource technology, 2012-03, Vol.108, p.162-168

Ort / Verlag

England: Elsevier Ltd

Erscheinungsjahr

2012

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• ► Flocculation was introduced into the industrial Saccharomyces cerevisiae PE-2 strain. ► The modified strain kept parental fermentation performance. ► The recombinant strain was applied to a repeated-batch system in an airlift reactor. ► Flocculation was efficiently applied for biomass recycling by cell sedimentation. ► An average of 142g/L ethanol was produced during a succession of 10 batches. The flocculation gene FLO1 was transferred into the robust industrial strain Saccharomyces cerevisiae PE-2 by the lithium acetate method. The recombinant strain showed a fermentation performance similar to that of the parental strain. In 10 repeat-batch cultivations in VHG medium with 345gglucose/L and cell recycling by flocculation–sedimentation, an average final ethanol concentration of 142g/L and an ethanol productivity of 2.86g/L/h were achieved. Due to the flocculent nature of the recombinant strain it is possible to reduce the ethanol production cost because of lower centrifugation and distillation costs.